Salmonella Gallinarum, Fowl Typhoid



Introduction

Disease caused by one of the two poultry-adapted strains of Salmonella bacteria, Salmonella Gallinarum. This can cause mortality in birds of any age. Broiler parents and brown-shell egg layers are especially susceptible. Chickens are most commonly affected but it also infects turkeys, game birds, guinea fowls, sparrows, parrots, canaries and bullfinches. Infections still occur worldwide in non-commercial poultry but are rare in most commercial systems now.

Morbidity is 10-100%; mortality is increased in stressed or immunocompromised flocks and may be up to 100%. The route of infection is oral or via the navel/yolk. Transmission may be transovarian or horizontal by faecal-oral contamination, egg eating etc, even in adults. The bacterium is fairly resistant to normal climate, surviving months, but is susceptible to normal disinfectants.

Signs

·         Dejection.
·         Ruffled feathers.
·         Inappetance.
·         Thirst.

·         Yellow diarrhoea.
·         Reluctance to move.

Post-mortem lesions

·         Bronzed enlarged liver with small necrotic foci, and/or congestion.
·         Engorgement of kidneys and spleen.
·         Anaemia.
·         Enteritis of anterior small intestine.

Diagnosis

Isolation and identification. In clinical cases direct plating on Brilliant Green, McConkey and non-selective agar is advisable. Enrichment procedures usually rely on selenite broth followed by plating on selective media. Tube and rapid plate agglutination tests have been the standard serological tests for many years but have only been validated for chickens. LPS-based Elisa assays have been developed but not widely applied commercially. Differentiate from Pasteurellosis, pullorum disease and coli-septicaemia.

Treatment

Amoxycillin, potentiated sulponamide, tetracylines, fluoroquinolones.

Prevention

Biosecurity, clean chicks. As with other salmonellae, recovered birds are resistant to the effects of infection but may remain carriers. Vaccines for fowl typhoid have been used in some areas, both live (usually based on the Houghton 9R strain) and bacterins.



Introduction

Disease caused by one of the two poultry-adapted strains of Salmonella bacteria, Salmonella Pullorum, this usually only causes mortality in birds up to 3 weeks of age. Occasionally it can cause losses in adult birds, usually brown-shell egg layers. It affects chickens most commonly, but also infects turkeys, game birds, guinea fowls, sparrows, parrots, ring doves, ostriches and peafowl. It still occurs worldwide in non-commercial poultry but is now rare in most commercial systems.

Morbidity is 10-80%; mortality is increased in stressed or immunocompromised flocks and may be up to 100%. The route of infection is oral or via the navel/yolk. Transmission may be transovarian or horizontal mainly in young birds and may sometimes be associated with cannibalism. The bacterium is fairly resistant to normal climate, surviving months but is susceptible to normal disinfectants.

Signs

·         Inappetance.

·         Depression.
·         Ruffled feathers.

·         Closed eyes.

·         Loud chirping.
·         White diarrhoea.
·         Vent pasting.
·         Gasping.
·         Lameness.

Post-mortem lesions

·         Grey nodules in lungs, liver, gizzard wall and heart.
·         Intestinal or caecal inflammation.
·         Splenomegaly.
·         Caecal cores.
·         Urate crystals in ureters.

Diagnosis

Isolation and identification. In clinical cases direct plating on Brilliant Green, McConkey and non-selective agar is advisable. Enrichment procedures usually rely on selenite broth followed by plating on selective media.

Differentiate from Typhoid, Paratyphoid, paracolon, other enterobacteria, chilling and omphalitis

Treatment

Amoxycillin, poteniated sulponamide, tetracylines, fluoroquinolones.

Prevention

Eradication from breeder flocks. As with other salmonellae, recovered birds are resistant to the effects of infection but may remain carriers. Vaccines are not normally used as they interfere with serological testing and elimination of carriers.





Introduction

Salmonella Enteritidis and S.Typhimurium are presented separately from other sero-types of Salmonella because, on the one hand, these bacteria are often specifically cited in zoonosis control legislation, and, secondly, because there are differences in the epidemiology as compared to other salmonellae. These are the predominant sero-types associated with human disease in most countries.


Salmonella Enteritidis, especially phage type 4, has become much more common in both poultry and humans since the early 80s. The prevalence of S.Typhimurium has remained relatively stable though the spread of the highly antibiotic-resistant strain DT104 in various farmed species gives some reason for concern. Infections in chickens, turkeys and ducks cause problems worldwide with morbidity of 0-90% and a low to moderate mortality. Many infected birds are culled and others are rejected at slaughter. The route of infection is oral; many species are intestinal carriers and infection may be carried by faeces, fomites and on eggshells.

Vertical transmission may be either by shell contamination or internal transovarian contamination of yolk. Feed and feed raw material contamination is less common than for other sero-types. The bacteria are often persistent in the environment, especially in dry dusty areas, but are susceptible to disinfectants that are suitable for the particular contaminated surfaces and conditions, applied at sufficient concentrations. Temperatures of around 80°C are effective in eliminating low to moderate infection if applied for 1-2 minutes. This approach is often used in the heat treatment of feed. Predisposing factors include nutritional deficiencies, chilling, inadequate water and other bacterial infections.

Signs

·         Dejection.
·         Ruffled feathers.
·         Closed eyes.
·         Diarrhoea.
·         Vent pasting.
·         Lost appetite and thirst.
·         Stunting in older birds.

Post-mortem lesions

·         In acute disease there may be few lesions.
·         Dehydration.
·         Enteritis.
·         Focal necrotic intestinal lesions.
·         Foci in liver.
·         Unabsorbed yolk.
·         Cheesy cores in caecae.
·         Pericarditis.
·         Perihepatitis.
·         Misshapen ovules in the ovaries in S.E. infection

Diagnosis

Isolation and identification. In clinical cases direct plating on Brilliant Green and McConkey agar may be adequate. Enrichment media such as buffered peptone followed by selective broth or semi-solid media (e.g. Rappaport-Vassiliadis) followed by plating on two selective media will


greatly increase sensitivity. However this has the potential to reveal the presence of salmonellae that are irrelevant to the clinical problem under investigation.

Differentiate from Pullorum/Typhoid, other enterobacteria such as E. coli. S.Enteritidis causes cross-reactions which may be detected with S.Pullorum serum agglutination tests. It is possible to detect reactions with specific antigens in agglutination tests but competitive and direct Elisa tests are more commonly used today.

Treatment

Sulphonamides, neomycin, tetracyclines, amoxycillin, fluoroquinolones in accordance with the sensitivity. Good management. Chemotherapy can prolong carrier status in some circumstances.

Prevention

Uninfected breeders, clean nests, fumigate eggs, all-in/all-out production, good feed, competitive exclusion, care in avoiding damage to natural flora, elimination of resident infections in hatcheries, mills, breeding and grow-out farms. Routine monitoring of breeding flocks, hatcheries and feed mills is required for effective control. Early depletion of infected breeding stock is required in some countries such as those of the European Union. Infection results in a strong immune response manifest by progressive reduction in excretion of the organism and reduced disease and excretion on subsequent challenge.

Vaccines are increasingly being used for S. Enteritidis and S. Typhimurium infection; both inactivated (bacterins) and attenuated live organisms.

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